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Susanna Bachle

Susanna Bachle is an Outreach Scientist at Addgene interested in Open Science, Global Health and organizing events inside and outside Addgene. You can follow her on Twitter @SusannaMBachle.

Recent Posts

Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

Posted by Susanna Bachle on Mar 17, 2020 9:32:55 AM

David Liu’s lab created the first base editor in 2016 (Komor et al., 2016) and since then has been trying to expand their precision editing capabilities. Base editors make specific DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case a deaminase. Base changes from C•G-to-T•A are mediated by cytosine base editors (CBEs) and base changes from A•T-to-G•C are mediated by adenine base editors (ABEs). How does this work? Through molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the Cas domain directs the modifying enzyme to the target site, and the deaminase induces the DNA base change without a DNA double-strand break. But base editors aren’t perfect. They may be slow, can only target certain sites, or make only a subset of base substitutions. 

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Topics: CRISPR, Base Editing

Three Key Considerations For Precise Neuronal Targeting Using AAV Technologies

Posted by Susanna Bachle on Nov 27, 2019 9:03:45 AM

Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience with many brain regions, neuronal networks, and specific neuronal populations to consider. To tease apart how different neurons impact a certain output, neuroscientists have begun to use more precise genetic tools: activators, inhibitors, sensors, recombinases, and more. With the help of adeno-associated viral vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation of specific neuronal subtypes and determine how these changes affect the phenotype. Viral vectors and genetic approaches are continuously refined and developed so there are many combinations to choose from for your experiments!

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Topics: Viral Vectors, Viral Vector Protocols and Tips, AAV

Microbes: Look, Listen, and Tell

Posted by Susanna Bachle on Aug 28, 2018 8:04:13 AM

When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs listening to talks all day. But nope, not at the American Society for Microbiology 2018 Microbe meeting. Soon after I arrived, I was looking through a paper-based, affordable, and portable microscope called the ‘foldscope’ (Dr. Prakash, Stanford University). Right there on the floor outside the lecture hall.

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Topics: Microbiology, Other

Popular Retroviral Vectors and Their Uses in Scientific Research

Posted by Susanna Bachle on Jul 24, 2018 8:51:21 AM

Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make excellent tools for genetic engineering. There are several different viral vectors to choose from, for example gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments best have a look at this viral vector overview.

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Topics: Viral Vectors, Retroviral and Lentiviral Vectors

Transferable Skills Guide: External Collaborations

Posted by Susanna Bachle on Apr 19, 2018 9:25:44 AM

A recent survey of PhDs found that many researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where you might need some help. Today in our transferable skills guide: Collaborating with others outside your organization also known as external collaborations.

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Topics: Science Careers, Professional Development

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