Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair (HDR), the pathway for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous end joining (NHEJ), HDR occurs at a relatively low ...
Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available in the Addgene viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage and a blanket, then scroll on for some wintery delights!
Over 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database. Previously developed genome editing methods using nucleases and base editors have the potential to correct only a minority of those variants in most cell types. But ...
Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.
Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy in research, but we’re really borrowing these tools from Mother Nature’s toolbox. Natural plasmids, also called environmental plasmids, are all around us!
If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check out the 34 new preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1 serotype.
If cloning methods had personalities, SLIC (sequence- and ligation-independent cloning) would be a true rebel. Not only does this system not use site-specific recombination, it also doesn’t require a ligation step. Based on the robust system of homologous recombination found in ...
Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.
