Multiple Authors

Blog articles by Multiple Authors

A cartoon schematic of prokaryotic chromosomal replication. The parent cell DNA is shown as a circular chromosome with a small region highlighted as the origin of replication, or ori.   During replication, the DNA helix has separated at the ori, creating a “bubble” of two single strands of DNA. The point of separation of the helix into these single strands is the replication fork. Two replication forks form on either end of the ori. At each replication fork, a helicase processively separates the DNA strands, and a polymerase synthesizes a new DNA strand paired to each single parent strand.  After termination, which is not shown in detail, the process results in two identical daughter cell DNA chromosomes.
A chart with MoClo kit recommendations for different modal organisms and applications. 
 A screenshot of the Addgene home page with arrows pointing at Online Submission and Spreadsheet Submission on the Deposit drop down menu.
Photo of iodixanol gradient after ultracentrifugation; and a cartoon showing an iodixanol gradient after ultracentrifugation.

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