The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus packaging methods. Across these methods, the following components are needed:
There are several facilities nationwide where you can obtain excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in your own lab with standard molecular biology tools and tissue culture experience. Here, we’ll go over the basics of rAAV ...
What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 20 additions we've made to our viral vectors repository since March.
All plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA replication is initiated at the ori and may or may not be synchronized with the replication of the ...
Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.
If you are job searching, you may be wondering if you need to go to the effort of writing a cover letter. After all, they’re less common than they were ten years ago, and it does take a bit of time to craft one. We still think they're useful, so we've updated our cover letter ...
Modular cloning is a popular DNA assembly tool used to build single- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free expression systems, mammalian cells, yeast, flies, and even plants! In this blog we will review common ...
Starting today, you may notice some changes to our website! Part of sharing science and making access to materials easier is improving the tools you use to share and access them. Addgene’s product, development, and content teams have made some changes to the look and feel of ...
