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Kendall Morgan

Kendall is a science writer based in North Carolina. She has a PhD in Biology from the University of Oregon and a certificate in science writing from the University of California, Santa Cruz. She writes about science, medicine and science culture and is dedicated to making it easier for scientists to share what they make and do with each other and the world.

Blog articles by Kendall Morgan

CRISPR

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Kendall Morgan December 10, 2020

Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel. Adenoviral vectors (AdVs) have been used for many years for in vivo delivery and gene therapy and represent one of the most studied viruses. Adenoviruses are non-enveloped double-strand DNA viruses ...
Schematic of targeted gene knock-out (left) and targeted DNA knock-in (right) by CRISPR/Cas9. Adenoviral CRISPR vectors (left) or CRISPR vectors and donor DNA vectors (right) are transduced to target cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final step of targeted gene knock-out (left), a fragment is deleted from two homologous strands of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA knock-in, donor DNA is copied into the cut site through error-free homologous recombination (HR).
Plasmid Elements

Plasmids 101: Origin of Replication

Kendall Morgan November 11, 2020

Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of any plasmid: the origin of replication/replicon. The replicon is comprised of the origin of replication (ori) and all ...
The Origin of Replication
Cancer

Modulate the Activity of 17 Signaling Pathways with One Kit!

Kendall Morgan March 22, 2016

When cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable but fleeting. “What’s been shown by others is that, in a relatively short amount of time, cancers become resistant to drugs, particularly targeted therapies,” said ...
Addgene News

Donations from Addgene to Yield Answers for Rare Disease Researchers

Kendall Morgan September 15, 2015

Every year, the Rare Genomics Institute sponsors a global competition for researchers working on rare diseases, offering them the chance to win some of the latest tools and technologies in life sciences research. The rare disease research community has certainly noticed: This ...
CRISPR

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Kendall Morgan July 28, 2015

As Eric Mendenhall of the University of Alabama in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding portion of the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty member) have ...
Synthetic Biology

CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Kendall Morgan April 22, 2015

As Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical methods to push the scale of genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering on a genomic ...
CRISPR

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Kendall Morgan February 24, 2015

CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind. “We had not managed to create a system to allow us to ...

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