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Jason Nasse

Jason Nasse is a senior scientist at Addgene specializing in AAV vectors and neuroscience. He obtained his Ph.D. in neuroscience from The Ohio State University, and is interested in helping scientists utilize viral vectors in their experiments and promoting open-data sharing through online data repositories.

Blog articles by Jason Nasse

Optogenetics

Viral Vectors 101: Optogenetic Tools

Jason Nasse June 27, 2023

In 2005, Boyden et al. described the first light-activated tool for controlling neuronal activity, channelrhodopsin-2 (ChR2), a blue light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to blue light, this channel activates neuronal ...
Cartoon showing an experimental setup for using an optogenetic tool on a mouse neuron in an in vivo model.
Viral Vectors

Viral Vectors 101: AAV Variables That Matter

Jason Nasse June 1, 2023

Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst. While it is tempting to just dive right in and start injecting your virus, a successful AAV experiment starts with validation and optimization. Although there are different ...
Flow charts show steps for selecting serotype and promoter on top, and steps for testing serotype, titer, and promoter in the middle. On the bottom are three unconnected steps listing how to test serotype, titers, and promoters
Open Science

Negative Can Be Positive: Open AAV Data with Addgene

Jason Nasse September 6, 2022

Working with AAV vectors in the brain and peripheral nervous system presents a special challenge to scientists. What works well in one cell or neuron type may not work well for a neighboring cell type, even within the same brain region or nucleus. Just optimizing AAV expression ...
Survey results showing scientists want more negative data in the AAV data hub
Viral Vectors

A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

Jason Nasse November 2, 2021

Many neuroscience experiments that require gene expression in a specific cell type rely on transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent AAV vectors for selective transgene expression. While this is a powerful ...
Schematic of Plasmid Amplification in Recombinase Deficient e. Coli

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