Beth Kenkel is currently a research scientist at a cell therapy company. She is particularly interested in science communication and viral vectors. Follow Beth on twitter @ElizabethKenkel.
We’re excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief Human Resource Officer for Harvard Medical School. The addition of Cicalese to the Board of Directors brings human resources experience and insight to the now 13 member board ...
I didn’t plan to get a master’s degree. I wanted to be a scientist and you need a PhD to be a scientist, or so I thought. So I entered a molecular and cellular biology PhD program in 2011, which I left four years later with a master’s. At the time my degree felt like a ...
Your next cool experiment requires some AAV. Where do you start? Plasmids of course! You just need three plasmids to start making the AAVs you need for your experiment: the packaging plasmid which contains the AAV structural and packaging genes, the adenoviral helper plasmid ...
Optogenetics is a neuroscience method that lets you fire neurons with the flick of a light switch. Neurons are not typically persuaded to fire when light is shined on them, but the expression of light-gated ion channels such as channelrhodopsins (ChRs) makes them ...
Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging capacity is limited to ~4.7kb, or roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene will fit within this limit, some like prime ...
So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before, but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making AAV starts with some plasmids. You just need three ...
Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing, activating gene expression, repressing gene expression, and much more. But there’s one thing ...
Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this opportunity and are excited for this next step. But wait, now you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready for this ...
