CASTing Off for New Shores in Human Genome Editing

By Emily P. Bentley

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A cartoon plasmid at the center of the image has arrows leading to many possible host species: gram negative bacteria, gram positive bacteria, yeast, and mammalian cells.
A cartoon depiction of a lentiviral genome forming an episome in the presence of non-functional LV integrase. The linear lentiviral genome has a long terminal repeat (LTR) on each end. A split arrow shows two paths for circular episome formation. Homologous recombination within the LTRs results in a single LTR in the circular episome. Non-homologous end joining results in two adjacent LTRs in the circular episome.
Fingers picking up biological samples in a glass vial.

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