By Amanda Hazen
Read More
Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant protein that will be produced. Many ...
In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds, thousands, or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution stock of the ...
Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy in research, but where did these tools come from and why do they exist in nature? Read on to learn more about environmental plasmids, and how they’ve helped us develop ...
When facing a cloning project, scientists are no longer limited to traditional restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given set of resources, time, and experimental needs. Since its invention in the late ...
If you’re into cloning, you’re probably aware that there are several methodologies currently available for approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning ...
One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout", the gene by replacing it or disrupting it with an piece of DNA designed in the lab. Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila ...
Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to ...
