By Tyler Ford
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Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or ...
This post was contributed by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision medicine seems so simple! Take a patient’s tumor sample, use cutting edge genomic technologies to map the mutations ...
Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect a protein of interest’s function. This technique relies on the generation of a plasmid library that expresses all desired variants of a protein. Applying a selective pressure ...
Hopefully you know that, if you’re an academic researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections of plasmids as kits with greatly reduced costs per ...
This post was contributed by guest bloggers Keith Pardee and Alexander A. Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and, for the most part, had been associated with low morbidity and mild symptoms. This changed in ...
This post was contributed by guest blogger Nathaniel Roquet, a PhD student in the Harvard Biophysics program and researcher in the Lu Lab at MIT. Note: The following blog post reduces the content of our paper, “Synthetic recombinase-based state machines in living cells” (1), ...
This post was contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. It is commonly cited that approximately one-third of cellular proteins are modified through phosphorylation (1). However, the expansion ...
