This post was contributed by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated cells into induced pluripotent stem cells (iPSCs) by the ...
In the fourth episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly newsletter or on our hot ...
In the third episode of our Hot Plasmids podcast series, you'll learn about optogenetics tools for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors, DIY DNA ladders. You can find additional hot plasmids in our newsletter or ...
In the second episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids in our newsletter or on our hot plasmids webpage.
Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 and pPSU2. When restriction digested with PstI or ...
This post was contributed by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad Institute of Harvard and MIT. The notion of cancer precision medicine seems so simple! Take a patient’s tumor sample, use cutting edge genomic technologies to map the mutations ...
Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect a protein of interest’s function. This technique relies on the generation of a plasmid library that expresses all desired variants of a protein. Applying a selective pressure ...
Hopefully you know that, if you’re an academic researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections of plasmids as kits with greatly reduced costs per ...
