By Rachel Leeson
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It’s not just the antibodies that matter when you’re prepping for your experiment - there are a number of outside factors that need to be considered as well. In this post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods towards ...
If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies in your lab. Perhaps you’re even in that mythical place where antibodies are well-organized, documented, and easy to find (though we might need to see it to believe it.) As ...
Immunofluorescence (IF), is an immunoassay that brings to light the cellular world. The technique allows you to ask questions like: “Where does my protein of interest live within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact ...
There are a lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of an antibody isotype? An isotype determines several key characteristics of an antibody as well as the role it plays in an immune response. But ...
Antibodies are used in many different experiments that require scientists to detect proteins in their samples. One technique that relies heavily on antibodies is ELISA, which stands for Enzyme-linked Immunosorbent Assay. ELISA are used to detect proteins within a 96- or ...
Have you ever wanted to measure expression of your protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex population. Have you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting ...
When you’re searching for an antibody to use in your next experiment, you’ll probably notice a lot of options to choose from. In this article we’ll cover polyclonal antibodies, one of the many different types of antibodies available (others you’ll encounter include monoclonal ...
