Antibodies 101: Conjugation

By Ashley Waldron

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Graphic showing in vitro models for immunogenicity prediction of therapeutic antibodies
flow plot with two populations
Cartoon showing the different types of controls necessary for a microscopy experiment. Full details are in alt text in post.
Schematic summarizing the problem with species on species immunohistochemistry. The schematic is titled Species on Species Staining - The Problem and shows a cell membrane with a target protein and an endogenous IgG. A primary antibody is bound to the target and secondary antibodies conjugated with fluorophores are bound to both the primary antibody and the endogenous IgG.
Diagram of HIS-tagged protein column purification

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