By Ashley Waldron
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You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you need are some of your favorite proteins purified to carry out your plan. With a little thoughtful planning, affinity tags can make protein purification a cinch. Types of ...
Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue type, such as PBMCs or tumors, and moreso than other methods, the identification and isolation of rare cell types. In ...
Expansion Microscopy (ExM) promises an easier, more accessible way to image biological features previously only visible via techniques like super-resolution microscopy or electron microscopy. Since its introduction in 2015 by the Boyden Lab, ExM has been steadily growing in ...
Addgene is excited to announce the launch of our partnership with the Institute for Protein Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of ...
IBEX is a highly multiplexed tissue imaging method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading the tissue (Radtke et al., 2020, 2022). I do mean highly multiplexed: you can image over 65 different parameters using ...
You may be familiar with immunofluorescence (IF, often referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where an antibody binds a target protein in your sample, then a fluorescently labeled secondary antibody ...
When it comes to using antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc, domain. Sure, we all know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies to bind. We also ...
