By Mary Gearing
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This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut it for bioinformatics. All kinds of biologists are finding themselves using computational approaches to analyze large data sets (myself included) and we are faced with finding ...
Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase (available from a licensed distributor) allow you to quickly transfer a DNA sequence from ...
This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your stab from Addgene, streak for single colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in ...
Born to a family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best of the best students in his home country. He was selected as one of 600 scholars among 100,000 eligible to attend the University of Malawi where he focused on agricultural ...
Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR ...
This post was written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens the time needed to go from planning an experiment to performing one. At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade ...
Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish the same thing in less time and for less money. The key steps to colony PCR are: 1) ...
