Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using antibodies to capture protein:DNA complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ...
A 2017 survey found that many researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting that researchers learn many transferable skills while working in the lab and by offering advice on areas where ...
Selectable transgene markers for plant transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings or callus (undifferentiated plant cell culture), ...
You've been following along on our journey to name our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution launch - and we're excited to say our long naming journey is at an end! Their name is...(drum roll, please!)...
Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If ...
The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg lab (also called the ...
What is the universal thing that unites all scientists? Curiosity? Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of an experiment failing because of a small, avoidable mistake. Or worse, when you're trapped in a pattern of ...
