Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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This post was originally written by Tyler Ford in 2018. It was updated by guest blogger Abhi Aggarwal in 2022. Recent updates to iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here, we look at the origins of the system and explore ...
Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed through NABOR are recombinant antibodies expressed from plasmids that have been deposited with Addgene. Here, ...
FLIS and Flies Drosophila, more commonly known as fruit flies, have been an important animal model for research, particularly genetics research, for decades. Its short life cycle, reasonably close genetic homology to humans, and small size have allowed scientists to use it as a ...
Addgene is thrilled to announce the launch of our latest community resource: Recombinant Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order ready-to-use antibodies for your experiments directly from Addgene.
Your new antibody has arrived, hooray! Before you jump straight into the exciting new experiments that you’ve been planning, it is a good idea to pause and make sure that your antibody has been appropriately validated so that you can have confidence in your results. But what is ...
Picture this: you’ve been assigned an exciting new project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background papers you could get your hands on, formulated a hypothesis, and planned out your key experiments. Unsurprisingly, many ...
Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using antibodies to capture protein:DNA complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ...
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