By Joanne Kamens
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Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked this up after trying out the new protocols.io beta platform for sharing, annotating, and storing life science protocols. Since we began sharing this protocol on the ...
Scientists are excited by somewhat unusual things. For example, I am not that interested in meeting movie stars, but when I met the astronaut Jim Lovell I was speechless (the club of people who have been in space is pretty small). Therefore, I was delighted to be invited to the ...
Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple or bidirectional promoters, or the creation of bicistronic or ...
This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists must do science to be happy. What do we have to change to ensure that all scientists can have successful careers doing science in some form? There is a lot of ...
Congratulations, you have a plasmid expressing your gene of interest (YGOI) and are ready to dive into your functional experiments! Whether you’ve cloned the plasmid yourself or obtained it from a colleague down the hall, it is always a good idea to take some time to confirm ...
This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well in part that’s what leaving academia feels like for a lot of researchers. For many years I struggled with the questions of “Will I leave academia?” and "What type of science ...
Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The ...
