By Joanne Kamens
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Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple or bidirectional promoters, or the creation of bicistronic or ...
This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists must do science to be happy. What do we have to change to ensure that all scientists can have successful careers doing science in some form? There is a lot of ...
Congratulations, you have a plasmid expressing your gene of interest (YGOI) and are ready to dive into your functional experiments! Whether you’ve cloned the plasmid yourself or obtained it from a colleague down the hall, it is always a good idea to take some time to confirm ...
This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well in part that’s what leaving academia feels like for a lot of researchers. For many years I struggled with the questions of “Will I leave academia?” and "What type of science ...
Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The ...
Mark Howarth’s lab at the University of Oxford is dedicated to generating new tools to manipulate biology based on molecular features found in nature, with the ultimate goal to improve the diagnosis of disease, and cancer in particular. They recently introduced the ...
There is essentially no better place for a scientist to make new relationships than at scientific conferences. Conferences provide the opportunity to meet people who are interested in the same things you are on a deep level. Right away you have something in common. Namely, the ...
