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Michael G. Lemieux

Michael G. Lemieux is a Senior Scientist at Addgene with interests in professional development, scientific education, and molecular biology.

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A Guide to Designing a Scientific Poster: Content, Layout, and More

Posted by Michael G. Lemieux on Oct 22, 2019 8:20:00 AM

You’ve done great research and made interesting discoveries. You’ve analyzed the data and generated beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference, you need to first make your poster. While a poster generally contains the same sections as a primary research article, it’s important to understand that presenting your work in poster format differs in many ways from writing a manuscript.

If you have ever attended a scientific conference, or even a lab recruiting session at your university, you are likely aware of how different posters can be. You have also probably thought about why certain posters resonate with you (or not). It is important to reflect on these impressions when thinking about crafting your own poster.

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Topics: Science Careers, Conferences

A new platform for fast functional characterization of potentially cancerous mutations

Posted by Michael G. Lemieux on May 7, 2019 8:39:32 AM

Thousands of mutations have been implicated in cancer. Yet, the majority of them remain uncharacterized. This represents a considerable barrier to furthering research and developing effective treatments for the disease. Recently, Kenneth Scott’s lab at Baylor College of Medicine and Gordon Mills’s lab at the MD Anderson Cancer Center tackled this problem by developing a moderate throughput functional genomic platform to annotate genetic perturbations that may play significant roles in cancer pathogenesis.

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Topics: Cancer

Plasmids 101: 5 factors to help you choose the right cloning method

Posted by Michael G. Lemieux on Aug 21, 2018 8:31:59 AM

You’ve spent days and weeks thinking of an amazing project. You’ve written your protocols, designed your experiments, and prepared your reagents. You’re going to engineer the best thing since CRISPR; you are ready to clone! But...how?

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Topics: Plasmids 101, Plasmid Cloning, Plasmids

Transferable Skills Guide: Career Planning Resources

Posted by Michael G. Lemieux on Nov 14, 2017 9:00:00 AM

recent survey of PhDs found that many researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where you might need some help. Today: Career Planning Resources.

The key to ensuring success during graduate school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc forever, and you need to be thinking about what comes next. To that end, one of the most important things that you can do now is diversify. Read on for an overview of some of the resources you can use to better prepare for your career, and increase your chances of landing that next coveted job!

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Topics: Science Careers, Professional Development

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Posted by Michael G. Lemieux on Dec 8, 2016 10:30:00 AM

If you’re into cloning, you’re probably aware that there are several methodologies currently available for approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others. Each method is unique and relies on specific components that are key to the cloning reaction. Understanding the specific components is essential for choosing the correct cloning method for your own experiments, and here we will focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what role does it play in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning experiments a little easier.

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Topics: Plasmids 101, Plasmid Cloning, Plasmids

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