This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although we only walk one path at a time, the variety of paths in life is mind-boggling. Unlike a mountain – with many routes up and only one destination – a career in the sciences ...
This post was contributed by guest blogger Iris Lindberg, Professor at the University of Maryland School of Medicine. In the Lindberg Lab we often make cell lines that overexpress genes of interest; more recently we have also been using Addgene CRISPR vectors to generate cell ...
This post was contributed by guest blogger Lydia Morrison from New England Biolabs. What is DNA assembly? In the context of cloning, DNA assembly refers to a method of physically joining multiple fragments of DNA to create a synthetically designed DNA sequence. There are ...
This post was contributed by guest bloggers Dominik Paquet and Dylan Kwart from Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne’s lab at the Rockefeller University in NYC. The CRISPR/Cas9 system is a versatile tool for precise gene editing in many organisms and ...
This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ...
This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate expression of your favorite gene (YFG) in flies. Read the first post here. Once ...
This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. Do you have a gene of interest but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you find an ortholog in the Drosophila ...
This post was contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for Molecular Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming to design 5 guide RNAs (gRNAs) targeting each of ...
