Viral Vectors 101: Gamma-Retroviral Packaging Systems

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A flow chart showing the steps of rAAV production. Details in blog post.
 A viral particle enters a host cell, enters the host nucleus, is transcribed into RNA, and is translated into protein.
Cartoon schematic: a barcoded transfer plasmid and packaging plasmids are put in barcoded LentiX-293T cells. From this, an arrow points to virus particles with a barcode; an arrow from the barcode points to
Structure of a recombinant AAV vector, where two ITRs flank the gene cassette.
Cartoon depicting (a)

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