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Hot Plasmids - October 2020

Posted by Various Addgenies on Oct 13, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

Here's what you'll find in this post:

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Topics: Hot Plasmids, Other Plasmid Tools, Plasmids

Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Posted by Various Addgenies on Jun 2, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

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Topics: Hot Plasmids, Other Plasmid Tools, Plasmids

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Posted by Various Addgenies on Mar 3, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

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Topics: Hot Plasmids, Other Plasmid Tools, Plasmids

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Posted by Various Addgenies on Mar 19, 2019 9:31:17 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

 

 

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Topics: Hot Plasmids

22 Hot Plasmid Technologies from 2014

Posted by Joanne Kamens on Jan 6, 2015 12:21:53 PM

Updated mini-transposon vector for bacterial mutagenesis or gene targeting

Victor de Lorenzo's lab has engineered a modular mini-Tn5 vector that can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or other markers into a target genome. They did this by selecting the important elements from existing transposon and vector systems and creating an all-synthetic vector that included only the elements needed for function.

The lab validated this vector, called pBAM1, by conducting random mutagenesis in the soil bacterium Pseudomonas putida and demonstrate that they can successfully create GFP fusion proteins with a variety of genes across the genome. Although this tool was published in 2011, it was only recently made available through Addgene and we want to highlight it for use in your research.

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Topics: Hot Plasmids, Other Plasmid Tools

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