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This post was written by Abhi Aggarwal, from University of Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs) have become a vital tool in neuroscience research. These fluorescent proteins light up in response to calcium, which is more than just ...
This post was written by Alyssa Shepard and Angelo Nicolaci, a PhD student at Moffitt Cancer Center. Engineering usually calls to mind building things, lots of math, and maybe heavy machinery. But not all engineering is at a large scale — some is done on much smaller equipment ...
This blog post was written by Dr. Kutubuddin Molla, investigator at ICAR-Central National Rice Research Institute. When it comes to genome editing, CRISPR is a name that resonates with nearly every biologist, academic, and researcher. Among the most well-known CRISPR-associated ...
For most people, the most familiar source of bioluminescence is the firefly. Along with charming nighttime displays, these insects have contributed an important tool to the scientific community: luciferases, which have become common genetic reporters and alternatives to ...
Previously, we provided a general overview of the QC process at Addgene. All plasmids go through this same initial QC process using an Illumina MiSeq, but that’s not the only technology Addgene uses to ensure accuracy of deposited plasmids. To resolve QC issues, assembly issues, ...
This post was written by Alfonso Timoneda, with significant contributions from Robert Hurt and Mohamed Soufi. So you’ve learned about the Modular Cloning (MoClo) system and what it can do for you, and you’ve chosen one of the MoClo kits available through Addgene that suits your ...
You have just purified your plasmid and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA you have or how pure your sample really is?
