New and Upcoming Viral Vectors - December 2019

Posted by Maya Peters Kostman on Dec 19, 2019 9:40:37 AM


New Viral Vectors AddgeneSince the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory.

Our new AAVs include:

  • Optogenetics AAV
  • Control AAV
  • Biosensor AAV
  • And more!


Optogenetics

Optogenetics continues to supply neuroscience with ever more efficient tools for light mediated neuronal control and we are expanding our viral service to make these readily available to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent.

Plasmid Serotype Name
124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40
124650 AAV9 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1
55645 AAV5 pAAV-hSyn Con/Fon hChR2(H134R)-EYFP
26975 AAVrg pAAV-CaMKIIa-hChR2(H134R)-mCherry
26976 AAV5 pAAV-hSyn-hChR2(H134R)-mCherry
26973 AAVrg pAAV-hSyn-hChR2(H134R)-EYFP

 

Control AAV

Control AAV allow researchers to not only interpret their results, but also confirm expression in target cells. While the various AAV serotypes can be used to achieve different patterns of expression, researchers often need to determine these transduction patterns empirically for their own experiments. To this end, we offer specific control AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes.

See our Serotype Testing AAV inventory for the entire collection!

Plasmid Serotype Name
51502 AAV5 pCAG-FLEX-EGFP-WPRE
114471 AAV1, AAV5 pAAV-Ef1a-fDIO mCherry
99130 AAVrg pAAV-mDlx-NLS-mRuby2
112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP
27056 AAVrg pAAV-Ef1a-DIO EYFP
50457 AAV2 pAAV-hSyn-DIO-EGFP

 

Biosensor AAV

Calcium sensors

Tracking calcium dynamics that govern such activities as neurotransmitter release is vital in studying neural networks. We have expanded our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options are also available for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials, is now offered under control of the CAG promoter for ubiquitous expression. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors).

Plasmid Serotype Name
104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE
104493 AAVrg pGP-AAV-syn-FLEX-jGCaMP7b-WPRE
104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE
104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE
105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s

Neurotransmitter sensors: Dopamine and norepinephrine

Neuroscience research relies on accurate monitoring of neurotransmitters and we are excited to offer AAV for the newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different levels of apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049 offering medium apparent affinity.

Plasmid Serotype Name
113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h
113049 AAV9 pAAV-hSyn-GRAB_DA1m
123308 AAV9, AAVrg pAAV-hSyn-GRAB_NE1m

Glutamate sensors

iGluSnFR is a useful tool from Loren Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged into AAVrg.

Plasmid Serotype Name
106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S
Click Here to Find Viral Vectors at Addgene

Viral vectors coming soon!

These vectors should be packaged and available as AAV in the next few months. Email us or check our website to access them!

DREADDs

Plasmid Serotype Name
 44361  AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry  
 44362  AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry

Controls

Plasmid Serotype Name
50459 AAV1 pAAV-hSyn-DIO-mCherry
27056 AAVrg pAAV-Ef1a-DIO EYFP
114471 AAV5 pAAV-Ef1a-fDIO mCherry
112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP
50457 AAV2 pAAV-hSyn-DIO-EGFP

Recombinases

Plasmid Serotype Name
87306 AAVrg, AAV5 AAV pEF1a-DIO-FLPo-WPRE-hGHpA

 

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