New and Upcoming Viral Vectors - December 2019

Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory.

Our new AAVs include:

• Optogenetics AAV
• Control AAV
• Biosensor AAV
• And more!

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Optogenetics

Optogenetics continues to supply neuroscience with ever more efficient tools for light mediated neuronal control and we are expanding our viral service to make these readily available to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent.

Plasmid	Serotype	Name
124603	AAV9	pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40
124650	AAV9	pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1
55645	AAV5	pAAV-hSyn Con/Fon hChR2(H134R)-EYFP
26975	AAVrg	pAAV-CaMKIIa-hChR2(H134R)-mCherry
26976	AAV5	pAAV-hSyn-hChR2(H134R)-mCherry
26973	AAVrg	pAAV-hSyn-hChR2(H134R)-EYFP

Control AAV

Control AAV allow researchers to not only interpret their results, but also confirm expression in target cells. While the various AAV serotypes can be used to achieve different patterns of expression, researchers often need to determine these transduction patterns empirically for their own experiments. To this end, we offer specific control AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes.

See our Serotype Testing AAV inventory for the entire collection!

Plasmid	Serotype	Name
51502	AAV5	pCAG-FLEX-EGFP-WPRE
114471	AAV1, AAV5	pAAV-Ef1a-fDIO mCherry
99130	AAVrg	pAAV-mDlx-NLS-mRuby2
112677	AAV1, AAV2	pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP
27056	AAVrg	pAAV-Ef1a-DIO EYFP
50457	AAV2	pAAV-hSyn-DIO-EGFP

Biosensor AAV

Calcium sensors

Tracking calcium dynamics that govern such activities as neurotransmitter release is vital in studying neural networks. We have expanded our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options are also available for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials, is now offered under control of the CAG promoter for ubiquitous expression. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors).

Plasmid	Serotype	Name
104489	AAVrg	pGP-AAV-syn-jGCaMP7b-WPRE
104493	AAVrg	pGP-AAV-syn-FLEX-jGCaMP7b-WPRE
104496	AAVrg	pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE
104495	AAVrg	GP-AAV-CAG-FLEX-jGCaMP7s-WPRE
105714	AAV8	pAAV-Ef1a-fDIO-GCaMP6s

Neurotransmitter sensors: Dopamine and norepinephrine

Neuroscience research relies on accurate monitoring of neurotransmitters and we are excited to offer AAV for the newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different levels of apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049 offering medium apparent affinity.

Plasmid	Serotype	Name
113050	AAV9, AAVrg	pAAV-hSyn-GRAB_DA1h
113049	AAV9	pAAV-hSyn-GRAB_DA1m
123308	AAV9, AAVrg	pAAV-hSyn-GRAB_NE1m

Glutamate sensors

iGluSnFR is a useful tool from Loren Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged into AAVrg.

Plasmid	Serotype	Name
106174	AAVrg	pAAV.hSynapsin.SF-iGluSnFR.A184S

Viral vectors coming soon!

These vectors should be packaged and available as AAV in the next few months. Email us or check our website to access them!

DREADDs

Plasmid	Serotype	Name
44361	AAV1	pAAV-hSyn-DIO-hM3D(Gq)-mCherry
44362	AAV1	pAAV-hSyn-DIO-hM4D(Gi)-mCherry

Controls

Plasmid	Serotype	Name
50459	AAV1	pAAV-hSyn-DIO-mCherry
27056	AAVrg	pAAV-Ef1a-DIO EYFP
114471	AAV5	pAAV-Ef1a-fDIO mCherry
112677	AAV2	pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP
50457	AAV2	pAAV-hSyn-DIO-EGFP

Recombinases

Plasmid	Serotype	Name
87306	AAVrg, AAV5	AAV pEF1a-DIO-FLPo-WPRE-hGHpA

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 Additional resources on the Addgene blog

• Read all of our viral vectors blog posts
• Find tips for preparing AAVs
• Download our viral vectors 101 eBook

Resources on Addgene.org

• Learn about Addgene's viral service
• Find viral protocols and videos
• Find AAVs for your research