By Guest Blogger
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As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you have been in the cloning trenches yourself. Modular cloning, or MoClo, is a systematic cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning ...
Primer design. Plasmid mapping. DNA sequence analysis. We all have our favorite tools for tackling these particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular biology toolbox organized, today’s post features a list of free ...
We are excited to announce the fourth edition of our popular eBook, Plasmids 101, is now available!
If you’ve ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for designing your overhang sets. But are those rules the best way to design GGA overhang sets, particularly for high-complexity reactions?
If you study proteins, you’re probably quite interested in the canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find the available amino acids a bit…limiting. It may be time, then, to look towards genetic code expansion (GCE): ...
I was excited to see that Addgene has recently added a new feature for depositing labs to its website, allowing depositors to download their plasmid request data, as a CSV file, anytime. The CSV is a rich source of information and has data on the date, material, requesting PI ...
Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and off by binding proximal to their target genes. Since gene expression patterns determine everything from stem cell differentiation fate to tumor suppression (and most everything ...
