Plasmids 101 eBook 4th Edition

By Rachel Leeson

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Graphic showing the workflow for high complexity Golden Gate Assembly, starting with destination vector and PCR amplification of fragments, which combined in a single-tube reaction with enzyme and DNA ligase to produce the assembled DNA product.
Examples of gene, enhancer, and promoter traps at a genomic locus.
Repressible promoters Schematic-01
Steps of DNA transposon transposition

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