Antibodies 101: Multiplex Immunofluorescence

By Mike Lacy

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A carton schematic showing Fc effector functions
Cartoon of epitope tag applications
diagram showing creation of a chimeric antibody
Cartoon image of Abi in safety glasses in front of a conveyor belt with tubes of antibodies on it
Top (a): Graphic with a short orange rectangle with 6 Hs on it representing 6 Histidines attached on the right side to a long blue rectangle representing a protein. Below it, a long blue rectangle attached on the right side to a short orange rectangle with 6 Hs on it. Bottom (B) A series of long blue rectangles, each attached to a short orange rectangle on the right side. From top to bottom, the orange rectangles have 6, 7, 8, and 9 Hs, respectively.
3-step diagram showing (top) Step 1 - image of cells producing antibodies and next to it, Myc1-9E10 monoclonal antibody. (middle) Mcy1-E10 immnogen sequence (protein) AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA. (Bottom) Three proteins of various shapes, each with a Myc tag (represented by a gift tag) attached to them by a wire.

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