Antibodies 101: Introduction to Gating in Flow Cytometry

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Antibody with five example conjugation sites indicated. Each site represents a different conjugation strategy, 1) lysine 2) cysteine 3) carbohydrate 4) sequence modifications 5) protein-protein interactions.
flow plot with two populations
Cartoon showing the different types of controls necessary for a microscopy experiment. Full details are in alt text in post.

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