By Rachel Leeson
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If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options — or not know where to start looking to find one. Luckily, there are websites that can help: Antibody data repositories, which share validation data, and antibody search engines, ...
Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource for anyone looking to use antibody-based applications in their research.
Western blots can be tricky to get right. If you’ve thoughtfully done the technical design of your blot, but are still finding yourself having issues, you’re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting.
Antibody validation is to confirm (or refute) that the antibody is selectively detecting the target-of-interest in your assay and sample-of-interest. The approaches available broadly map onto the five pillars of antibody validation (see: Uhlen et al., 2016). In this post, we ...
Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you are not alone. Full-sized antibodies dominate the world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little ...
If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices to make when designing the experiment. What detection method? What membrane? What should you block with?
Now that you know how to read flow plots and have designed your first flow panel, you’ll load your samples into the cytometer and see one of two results for your antibody of interest: two clear populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk ...
