If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices to make when designing the experiment. What detection method? What membrane? What should you block with?
Now that you know how to read flow plots and have designed your first flow panel, you’ll load your samples into the cytometer and see one of two results for your antibody of interest: two clear populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk ...
What do a viral vector production facility, food allergy testing lab, and the grad student down the hall from you have in common? All of them rely on standard curves in their day-to-day work. Indeed, viral vector production facilities frequently use qPCR with a standard curve to ...
While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow for ...
Western blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what if you want to detect more than one protein in your sample? Or what if something weird happened during your western and your ...
An enzyme-linked immunosorbent assay (ELISA) is a versatile method used to quantify the level of target antigen in a sample. While Engvall et al. originally developed the ELISA assay to measure antibody levels, scientists have since adapted it for a host of different proteins ...
When it comes to labeling cells for flow cytometric analysis, the most common method is a cell surface label, where fluorophore-conjugated antibodies directly bind to epitopes of interest that are found in the extracellular space. The targeted epitopes can be motifs within ...
When you are running flow cytometry, you’ll need various controls to help you set up and analyze your samples. While you are probably familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as an application. These controls will ...
