Rachel Leeson

Rachel is a Science Communications Specialist at Addgene. She manages the blog and social media.

Blog articles by Rachel Leeson

Schematic of Fragmid assembly process from day one to day four and resulting vector architecture.  On day one fragment plasmids plus a destination vector are assembled using Golden Gate (BbsI). On day two the assembled vector containing a Guide (2xBsmBI), Promoter, N-terminus, Cas protein, C’terminus, and 2A-Selection undergoes an exonuclease V cleanup followed by transformation and plating. On day three two colonies per construct are picked, miniprepped and restriction digested for gel validation/whole plasmid sequencing. Below the assembled vector are examples of six vector architectures including pRDA_512 lentivirus, pRDA_722 lentivirus, pRDA_789 CROPseq lentivirus, pRDA_889 AAV, pRDA_575 Piggyback, and pRDA_791 empty plasmid.
Graphic representing the choices made in the technical design of a western blot.
Graph showing various categories of viral vector tools and what percentage each tool makes up of the Addgene viral vector item catalog.
addgenies smiling and talking together.

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