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Paolo Colombi

Paolo Colombi is currently the product development scientist at Addgene. In the lab he develops new assays and processes. Outside the lab, he enjoy the outdoor in all the possible aspects.

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Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

Posted by Paolo Colombi on Jul 28, 2020 9:15:00 AM

What if after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation? 

Great news. A subset of our collection has been prepared as part of our new cloning grade DNA service and is ready to use out of the box.

We estimate that providing cloning grade DNA could save scientists up to three days of work. We’ve taken care of all the steps going from streaking to a single colony to DNA extraction and quantification so that you can focus on what you want to do with your plasmid without spending time and resources in preparative experiments. 

We’re excited to begin offering this service to further contribute to our mission to “accelerate research and discovery.”

Here are the details.

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Topics: Addgene News, Plasmid Cloning, Plasmids

Plasmid Preps: Different Purity, Different Quantities, Different Uses

Posted by Paolo Colombi on Jun 16, 2020 9:25:46 AM

Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important to consider your experiment.  It will dictate the amount of DNA you need, and at which level of purity. Based on these premises we can classify a plasmid preparation in 3 different ways:

  • Transformation grade DNA
  • Cloning grade DNA
  • Transfection grade DNA
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Topics: Plasmid Cloning, Plasmids

What's the Best Way to Elute and Store Your Plasmid DNA?

Posted by Paolo Colombi on Jun 4, 2020 9:22:22 AM

If you use a kit for DNA purification or if you use a DIY purification protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols that recommend eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does it make a difference? Short answer: yes. This last step will influence the stability of your sample over time and determine which experiments you can effectively use the DNA.

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Topics: Molecular Biology Protocols and Tips, Plasmids

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