Paolo Colombi was formerly a product development scientist at Addgene. In the lab he develops new assays and processes. Outside the lab, he enjoy the outdoor in all the possible aspects.
What if after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your cloning experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation? Great news. A subset of our ...
Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important to consider your experiment. It will dictate the amount of DNA you need, and at which level of purity. Based on these premises we can classify a plasmid preparation in 3 ...
If you use a kit for DNA purification or if you use a DIY purification protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols that recommend eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does ...
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