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Michelle Cronin

Michelle Cronin was formerly the Technical Support Team Manager at Addgene, with a background in neurobiology, molecular biology, and biochemistry.

Blog articles by Michelle Cronin

Fluorescent Proteins

Fluorescent Tagging of Endogenous Genes with SapTrap

Michelle Cronin October 12, 2017

Since the discovery of GFP over 50 years ago, the growing spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function of cellular systems. FPs have been used to track protein localization, cell structure, intracellular ...

SapTrapFigure1.png
Fluorescent Proteins

Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

Michelle Cronin April 25, 2017

Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression. However, one caveat of FP-protein fusions (FP-chimeras) is that they undergo normal protein turnover. FP-chimeras are ...

PA-FP Fig 1B.png
Plasmids 101

Plasmids 101: FLEx Vectors

Michelle Cronin April 28, 2016

In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can be used to induce site-specific recombination events, and that the orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or excise a DNA ...

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