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Karen Guerin

Karen Guerin is a Senior Scientist at Addgene. She started working with viral vectors as a graduate student. As an Addgenie, she focuses on AAV production and quality control.

Recent Posts

AAVs in Retinal Gene Therapy

Posted by Karen Guerin on Jul 31, 2018 8:55:07 AM

It was by serendipity that I got into the field of gene therapy, more specifically AAV-based retinal gene therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated viral vectors (AAVs) to treat an inherited retinal degenerative disease called Retinitis Pigmentosa. I quickly became fascinated by this emerging technology and its potential for the treatment of some genetic diseases.

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Topics: Viral Vectors, AAV

Adenoviral Vector Production and Troubleshooting

Posted by Karen Guerin on Jun 14, 2018 7:44:26 AM

Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells, and do not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune response after in vivo administration, which results in the death of transduced cells and loss of transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis and vaccination!)

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Topics: Viral Vectors, Viral Vector Protocols and Tips, Adenoviral Vectors

Transferable Skills Guide: Managing a Team

Posted by Karen Guerin on Jan 30, 2018 9:42:28 AM

A recent survey of PhDs found that many researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where you might need some help. Today in our transferable skills guide: managing a team.

You’ve just been promoted, congratulations! You’re now a manager with your own team! But what does it really mean to manage a team?

If your graduate school experience was anything like mine you didn’t think much about management skills during your scientific training. I never thought about management skills before being propelled into the position of manager. I barely had any experience being managed! The good news is that management skills can be taught, but it will take time so be patient and keep an open mind.

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Topics: Science Careers, Professional Development

AAV Vector Quality Control: Going the Extra Mile with NGS

Posted by Karen Guerin on Sep 12, 2017 9:44:59 AM

Reproducible data are key to science, so scientists are used to repeating experiments to confirm their findings. But no scientist wants to repeat an experiment because of poor reagent quality. To make sure our AAV vectors are of the highest quality, we undertake a rigorous quality control process - read on to learn more!

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Topics: Viral Vectors, Viral Vector Protocols and Tips, AAV

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