This post was updated on Nov 14, 2017.
CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome engineering tool. It is easy to use, functional in most species, and has many applications (see a review of CRISPR applications here). That said, SpCas9 is not the only game in town, and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations associated with SpCas9. A novel protein, Cas13a (previously referred to as C2c2), has several unique properties that further expand the CRISPR toolbox. We'll cover how Cas13a was identified, the structure and function of Cas13a with a focus on what makes this molecule unique, and the various applications of Cas13a.