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Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Posted by Mary Gearing on Jun 9, 2016 10:30:00 AM

THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED TO REPEAT THE RESULTS DISCUSSED BELOW

Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has already distributed this plasmid all over the world, and the question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins Cas9 or Cpf1 - keep reading to learn more!

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Topics: Plasmid Technology, Genome Engineering, CRISPR

Using Virus in Your Research - A Primer for Beginners

Posted by Leila Haery on Jun 7, 2016 11:09:27 AM


We’ve all had the feeling where it seems like we’re the only one in the room who doesn’t know about an important scientific principle.

Example Scenario

Important science person: ...and then we found out that it wasn’t a deoxynucleotide, it was a dideoxynucleotide!

Room full of important science people: (laughter in unison)

You: (nervous laughter)

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Topics: Viral Vectors

How to Keep a Lab Notebook for Bioinformatic Analyses

Posted by Guest Blogger on Jun 2, 2016 10:30:00 AM


This blog post was contributed by guest blogger Kate Palozola

Traditional lab notebooks just won't cut it for bioinformatics. All kinds of biologists are finding themselves using computational approaches to analyze large data sets (myself included) and we are faced with finding the best system to document these types of analyses and their results. We are adept at recording wet-lab experiments using a “traditional” lab notebook; however, keeping track of computation work comes with new sets of challenges. One challenge with computational analyses is to keep track of why you are doing what you are doing. Another common challenge is to keep track of what works, and what does not work. Careful documentation will keep you on task and will prevent you from getting lost in the wide word of informatics.

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Topics: Lab Tips

PiggyBac-ing Through the Genome Editing Field

Posted by Guest Blogger on May 31, 2016 11:30:00 AM

Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase (available from Transposagen or a licensed distributor) allow you to quickly transfer a DNA sequence from the transposon vector to one of many TTAA sequences distributed throughout the genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen.

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Topics: Plasmid Technology, Genome Engineering

Plasmids 101: Optimizing Plasmid Yields

Posted by Julian Taylor-Parker on May 26, 2016 10:30:00 AM

Most of the time, plasmid prepping is a breeze. You get your stab from Addgene, streak for single colonies, sub-culture, and prep with one of the many commercially available DNA prep kits or your lab's favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than enough DNA to proceed with most applications, such as PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield is sub-optimal? If you have already purifed your plasmid, you can try to concentrate the DNA using a speed-vac, ethanol precipitation, or other chromatographic methods. But wouldn't it be nice to avoid an extra concentration step? If you are consistently getting sub-optimal plasmid yields from your prep, you may want to consider optimizing your growth conditions. In this blog, we will outline many of the variables that could affect DNA yields and suggest steps to super-charge your plasmid preps.

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Topics: Lab Tips, Plasmids 101

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