By Eric J. Perkins
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Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow you to test the function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The ...
Once you've gotten to know what a plasmid is in general, it's time to take a look at some of its parts. Elements such as the antibiotic resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the ...
Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient process– just 1 out of 10,000 cells on average! Without some means of ...
