Latest Posts

All Posts

Screening for Successful Genome Editing with Digital PCR

Posted by Guest Blogger on Mar 30, 2017 10:30:00 AM

This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta.

If you’re like many researchers these days, you are ready to take (if you haven’t already) the plunge into the world of precision genome editing. When it comes time to (hopefully) validate successful mutation of your favourite gene, there are several different methods available. Thankfully, there are many great resources available to help guide you through the rough waters of mutation validation, such as this "CRISPR 101" post. Next-generation sequencing technologies are the gold standard but they remain cost-prohibitive for many labs, and are often impractical for small projects. Most researchers instead turn to so-called “mismatch nuclease” assays (e.g. Surveyor® or T7E1) for mutation detection. While these methods paved the way for mutation validation, we found these assays frustrating to work with, time consuming, and minimally informative. In this blog post, we’ll introduce digital PCR as an emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays that will be elaborated below

Read More >

Topics: Genome Editing, Plasmids

PiggyBac-ing Through the Genome Editing Field

Posted by Guest Blogger on May 31, 2016 11:30:00 AM

Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase (available from Transposagen or a licensed distributor) allow you to quickly transfer a DNA sequence from the transposon vector to one of many TTAA sequences distributed throughout the genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen.

Read More >

Topics: Genome Editing, Plasmids

Kiran Musunuru on the Newest TALEN Genome-Editing System

Posted by Kendall Morgan on Dec 10, 2013 10:13:00 AM

The goal of Kiran Musunuru's lab in Harvard's Department of Stem Cell and Regenerative Biology is to understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients to uncover new gene variants associated with conditions of interest, then studying those variants in model systems: either human cells or mice.

In a recent issue of Cell Stem Cell, Musunuru, Chad Cowan and their colleagues describe a much more efficient tool for doing that disease modeling work in human pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they could use their TALEN kit to quickly and efficiently generate human stem cells edited to carry mutant versions of 15 different disease-associated genes.

Addgene spoke to Musunuru about how the new kit works, the research the kit now makes possible, and how it compares to his CRISPR/Cas9 system.

Read More >

Topics: Genome Editing, Plasmids

Click here to subscribe to the Addgene Blog
 
Subscribe

 

All Topics

see all

Recent Posts