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Amanda Hazen

Amanda Hazen is a Scientist at Addgene. She enjoys helping the Addgene community by verifying the quality of the hundreds of plasmids distributed by Addgene everyday. Amanda also finds new ways to optimize lab processes and make plasmid distribution more efficient.

Recent Posts

Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

Posted by Amanda Hazen on Feb 1, 2018 10:07:31 AM

We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell and going into detail about how we use next generation sequencing results to perform quality control on deposited plasmids. We’ve also talked about how our new Snapgene generated maps provide improved feature detection with an easy to use interface. We regularly use Snapgene for our quality control process because of its expansive feature library and useful tools. In this blog, we’ll walk you through how a scientist at Addgene uses Snapgene to confirm the sequence of a plasmid and we’ll highlight some of the new features available on our website through our Snapgene powered maps and sequence analysis tools.

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Topics: Plasmids 101, Plasmid How To, Plasmid Cloning

Plasmids 101: An Inside Look at NGS Plasmid Quality Control

Posted by Amanda Hazen on Aug 3, 2017 8:49:45 AM

All plasmids coming through Addgene’s doors are now verified by next-generation sequencing (NGS) to provide you with more data. For most plasmids, we are now able to confirm the full insert and backbone sequence. In order to manage and interpret these data, we’ve updated our quality control workflow.  Here’s a peek at how our process has improved now that we’re backed by the power of NGS!

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Topics: Plasmids 101, Inside Addgene

Plasmids 101: Optimizing Plasmid Yields

Posted by Amanda Hazen on May 26, 2016 10:30:00 AM

This post was updated on March 21, 2018.

Most of the time, plasmid prepping is a breeze. You get your stab from Addgene, streak for single colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than enough DNA to verify your plasmid via sequencing or restriction digest.

Chances are, you’ll even have DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield is sub-optimal? If you are consistently getting sub-optimal plasmid yields from your prep, try our tips below!

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Topics: Lab Tips, Plasmids 101

Tips from the Repository Trenches: Using Barcodes to Track Samples

Posted by Amanda Hazen on Oct 6, 2015 10:30:00 AM

Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the journey of transformation and storage begins. Some samples are submitted as bacterial colonies in petri dishes, but close to 80% of samples are received as DNA in a microcentrifuge tube. Once a sample enters Addgene’s lab, a series of events is triggered. Each step is tracked through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database we use to keep track of and manage all of the samples that come through our doors. It would be a nightmare if we didn’t have a simple way to track all of our 40,000 samples available for distribution!

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Topics: Inside Addgene, Lab Tips

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