Plasmids 101: SunTag and Fluorescent Imaging

Posted by Mary Gearing on Mar 28, 2017 10:30:00 AM

Quick Announcement from the Plasmids 101 Team: In preparation for the release of Addgene's Fluorescent Protein eBook - our next couple of plasmids 101 posts will gain a healthy, fluorescent glow. Stay tuned for more fluorescence-based Plasmid 101 posts in the coming weeks!

In biology as in life, more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology, the Vale and Weissman labs took this biological lesson and created a system to amplify fluorescent signals. Named for the "stellar explosion SUperNova," SunTag can help you turn up the brightness in your fluorescent imaging experiments.

Read More >

Topics: Hot Plasmids, Plasmids 101, Fluorescent Proteins

27 Hot Plasmids from 2016

Posted by Tyler Ford on Dec 22, 2016 10:03:47 AM

Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over 45,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but be sure to let us know if you'd like to write about your plasmids in a future blog post.

Read More >

Topics: Hot Plasmids, News

The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

Posted by Jane Hannon on Nov 14, 2016 10:30:00 AM

All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly special. Over 1000 of our 45,000+ deposited plasmids have been requested more than 100 times each, thereby achieving the coveted “blue flame” status. We’re proud to honor the creativity, passion, and dedication to sharing epitomized by the creators of these plasmids with the first annual Blue Flame Awards.

Read More >

Topics: Hot Plasmids, Scientific Sharing, News

28 Hot Plasmid Technologies from 2015

Posted by Tyler Ford on Dec 23, 2015 10:30:00 AM



At Addgene we're continually impressed with the amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all of them attention they righlty deserve, but, in this post, we'll provide a small sampling of the many amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at blog@addgene.org and maybe we can write a blog post about it!

Read More >

Topics: Plasmid Technology, Hot Plasmids, Plasmid Kits

Illuminating Epigenetics with A FRET Based Biosensor

Posted by Emma Markham on Nov 19, 2015 10:30:00 AM

Epigenetics has recently been hitting the headlines, with sotires like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature. So what is epigenetics and what tools are available to study it?

Read More >

Topics: Hot Plasmids, Fluorescent Proteins, Techniques

Addgene blog logo

Subscribe to Our Blog