CRISPR 101: Making a Knock-In Cell Line

By Susanna Stroik

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Repressible promoters Schematic-01
Blugene in a labcoat reading a book next a gold-colored Blugene also reading a book.
three cartoon blugenes, one holding a plasmid, one holding a virus, and one holding a piece of DNA.
Cas family enzymes and their genome editing fast facts
Schematic showing MTRIA biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled receptors
Histogram of the frequency of SNPs mapped to targeting sequences of guides across 8 CRISPR libraries show cell lines of African ancestry have more guides affected than other cell lines. A second histogram of three corrected libraries shows the differences reduced in two cases and both peaks overlapped in the third.
The front page of the Addgene Help Center.

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