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Matt Ouellette

Matt Ouellette is a Senior Scientist on the Scientific Support Team at Addgene. He received his PhD at the University of Connecticut and studied at the Forsyth Institute as a postdoctoral researcher. Matt's interests include molecular biology, microbiology, and genetic engineering.

Blog articles by Matt Ouellette

Plasmids 101

Plasmids 101: Dimers and Multimers

Matt Ouellette January 10, 2023

When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut DNA control: a band or two appear on an agarose gel at notably higher molecular weights than expected, given the size of the plasmid. This is seen only in the uncut DNA; ...
On the left is a pMD2.G plasmid, 5822 bp, depicted as a circle with a CMV enhancer marked and arrows showing different plasmid components. On the right is a plasmid, pMD2.G Dimer, 11,644 bp, depicted as a circle with two CMV enhancers, and doubled plasmid components compared to the first image, represented by arrows.

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