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Kate Harten DeMaio

Kate Harten DeMaio is an Associate Scientist at Addgene. She has worked on the plasmid and viral vector teams at Addgene and she is happy to help share research tools with the scientific community.

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Droplet Digital PCR for AAV Quantitation

Posted by Kate Harten DeMaio on Jan 16, 2020 9:18:58 AM

Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the physical titer of our preps. We often repeat this assay to confirm that our results are accurate. But what if we could repeat this assay 10,000 times to ensure that our titers were as accurate as possible? This is where droplet digital PCR (ddPCR) comes in.

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Topics: Viral Vectors, Viral Vector Protocols and Tips, AAV

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