Important Considerations in Optogenetics Behavioral Experiments

Posted by Guest Blogger on Oct 1, 2015 10:30:00 AM

This post is part of our Primer on Optogenetics and was contributed by guest blogger Derek Simon.

The actual experiments you do will be determined by the topic you’re interested in studying, but, in today’s post, we’ll discuss some of the important considerations you should think about when developing optogenetics behavioral experiments. There are far too many behaviors that have utilized optogenetics to be fully summarized in a short blog post, but some examples I’m personally interested in include: intracranial self-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses on the neurobiology of addictive diseases, which means we are interested in circuits that mediate drug taking behavior. If a circuit reinforces behavior (activation of the circuit promotes subsequent, repeated activation), this is an approximation of reward or the sense of pleasure that the animal perceives through taking a drug. The ideal behaviors to test reinforcement are ICSS and place preference.

Read More >

Topics: Optogenetics, Lab Tips, Primer on Optogenetics

The Materials Science of Optogenetics Experiments

Posted by Guest Blogger on Sep 17, 2015 10:30:00 AM

This post is part of our Primer on Optogenetics and was contributed by guest blogger Derek Simon.

The surgeries and standard molecular neuroscience validation experiments we discussed last week are only half of the battle when using optogentics to answer a research question. The flip side of the optogenetics coin is materials science-based. Light is delivered to your opsin through a small piece of fiber optic cable implanted into the animal’s skull (right). The fiber optic cable is threaded throughand fixed to—an optical insulator called a ferrule (below). The fiber optic cable/ferrule is inserted into the target brain region using stereotaxic surgery and cemented to the animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic patch cable is then connected from laser to ferrule to deliver light pulses to the target brain region.

Read More >

Topics: Optogenetics, Techniques, Primer on Optogenetics

A Primer on Optogenetics: Introduction and Opsin Delivery

Posted by Guest Blogger on Sep 10, 2015 10:30:00 AM

This post is part of our Primer on Optogenetics and was contributed by guest blogger Derek Simon.

Optogenetics is spreading through the neuroscience community like wildfire and for good reason. For the first time in the history of neuroscience research we have a technology that allows us to show causality in neural circuits with incredible temporal and spatial precision (I’m not going to discuss the basic biology of optogenetics so if you are not familiar with it, these reviews are an excellent introduction [1-3] and openoptogenetics provides a variety of useful resources).

Read More >

Topics: Optogenetics, Primer on Optogenetics

Subscribe to Our Blog