Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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In complex metazoans, rapid cell division and large scale cell mobility are essential processes during embryonic development. These are required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated body. In contrast, these ...
Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue recombination was released. This blog has not been updated in response to this paper. Any hint of CRISPR editing in human embryos has been met with a storm of media coverage. ...
Luminescent molecules are very useful tools because we can easily detect and measure the light they emit. Proteins that give off light include chemiluminescent proteins, like luciferases, as well as fluorescent ones, like Green Fluorescent Protein (GFP). These molecules occur ...
Regulating translation is key to cellular function, especially during development or stress. With ribosome profiling, researchers have been able to study the effects of various stimuli on global translation, but a visual technique to study translation remained elusive. Two ...
This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ...
Fluorescent proteins have enabled scientists to pursue creative research avenues previously unavailable to them. With these fantastic tools it’s now easy to monitor protein expression, localization, and protein-protein interactions. Beyond these common applications, researchers ...
Signal transduction pathways are a lot like cell phone networks. Protein kinases deliver messages to the next members of their pathways, but where the kinases are located, their signal strength, and how long their signals last all impact transduction of the message. To study ...
