Latest Posts

All Posts

Find and Share AAV Data with Addgene's New AAV Data Hub

Posted by Melina Fan on Oct 8, 2019 8:33:52 AM

Dear scientists using AAV,

We hear your questions! How much virus should I inject? How long do I need to wait? What serotype and promoter should I use to target my favorite neurons?

We don’t know all of the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub. The Data Hub is an open platform for scientists using AAV to share practical information about their experiences. It will contain all of the juicy and important details that you won’t find in the final publication and we have already collected over 100 entries spanning six different species and dozens of different expression sites.

Read More >

Topics: Viral Vectors, Addgene’s Viral Service, Other Viral Vector Tools

New and Upcoming Viral Vectors - September 2019

Posted by Leila Haery on Sep 12, 2019 9:00:49 AM

Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory.

Our new AAVs include:

  • EGFP-expressing AAV for serotype testing
  • Calcium sensors and GABA sensors
  • Optogenetics
  • Recombinases
  • And more!
Read More >

Topics: Viral Vectors, Other Viral Vector Tools

New and Upcoming Viral Vectors - June 2019

Posted by Leila Haery on Jun 18, 2019 9:15:20 AM

Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory.

Our new AAVs include:

  • Control AAVs for serotype testing
  • jGCaMP7c for calcium detection
  • PSAM4 GlyR for chemogenetic inhibition
  • Flp expression for intersectional studies
  • And more!
Read More >

Topics: Viral Vectors, Other Viral Vector Tools

Neuronal labeling with Spaghetti Monster

Posted by Benoit Giquel on Aug 14, 2018 8:42:01 AM

The central nervous system (CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These functions and others are controlled by collections of neurons that are intricately wired into circuits through synaptic connections (Shepherd, 2004). Understanding the structure and function of these neural circuits is essential for neuroscience research. The use of genetic tools for visualizing and perturbing circuits together with the development of methodologies to deliver genes to the CNS have recently made it much easier to map these neuronal networks.

Read More >

Topics: Viral Vectors, Neuroscience, Other Viral Vector Tools

Using AAV for neuronal tracing

Posted by Klaus Wanisch on Aug 9, 2018 9:04:52 AM

Background on neuronal tracing

A key aspect to understanding the brain’s function is knowing its architecture, in particular the connections between different brain regions. For example, communication between the hippocampus and the prefrontal cortex brain regions is involved in the formation of episodic memory, a special type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional flow of information between different parts of the brain is mediated via individual neurons. Neurons are composed of a cell body, with dendrites receiving incoming information, and a projecting axon sending information onwards to other neuronal cells. Synapses at the terminals of axons form connections to dendrites of proximal neuronal cells. In the specific example of episodic memory, a subset of hippocampal neurons projects axons directly to the prefrontal cortex, but also indirectly via synapses to neurons in other brain regions. Further, the connections between regions are often reciprocal, forming a neuronal loop which is activated and strengthened during memory formation and memory retrieval.

Read More >

Topics: Viral Vectors, Cell Tracing, Other Viral Vector Tools

Click here to subscribe to the Addgene Blog
 
Subscribe

 

All Topics

see all

Recent Posts