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In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and Benjie Chen. We learn what it was like to get Addgene off the ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what it takes to start your own nonprofit, this is the episode for you.

If you’re doing high throughput work, it’s likely that you come into the lab wishing there was a machine that could make your life a hundred times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots that’ll take a little labor off our hands.

Good news! There is probably a machine or robot out there that does exactly what you want plus way more than you thought it could ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find out how you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG). There is so much to see and so many more robots than you ever knew existed!

As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find new ways to make lab processes more efficient. Being in the Boston area, we get many opportunities to learn from other biotech companies at workshops and can use what we learn to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs (NEB) hosted workshop at Lab Central and discuss why these types of workshops could be useful to anyone working in a lab.

In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds, thousands, or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and we thought it would be a good time to talk about the amplification and verification processes we use to ensure high-quality library distribution. You can also use these tips as a starting point when you need to amplify a library for your own experiments.

Reproducible data are key to science, so scientists are used to repeating experiments to confirm their findings. But no scientist wants to repeat an experiment because of poor reagent quality. To make sure our AAV vectors are of the highest quality, we undertake a rigorous quality control process - read on to learn more!