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Brook Pyhtila

Brook Pyhtila is a Scientific Curator at Addgene. She received her PhD at Stanford and did her post-doctoral work at Duke. She enjoys contributing to Addgene's database and web content so that scientists can more easily do their research.

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Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Posted by Brook Pyhtila on Mar 1, 2016 10:30:00 AM

Over the past decade, scientists have developed and fine tuned many different ways to clone DNA fragments which have provided appealing alternatives to restriction enzyme cloning. These newer technologies have become more and more common, and for good reason. They offer many advantages over the traditional restriction enzyme cloning we once relied exclusively on. In this blog post, I will go over some advantages, disadvantages, and examples of how scientists are using Gibson assembly to put together DNA fragments.

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Topics: Plasmid Technology, Plasmids 101, Plasmid Cloning, Protocols

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