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CRISPR 101: A New Series on Genome Editing & CRISPR-Cas

Posted by Marcy Patrick on Mar 5, 2015 12:06:12 PM

I am sure by now you have heard of CRISPRs. (If not, you can get up to speed here and here and here.) With such a fast moving technology, it is sometimes hard to keep pace with the new advances let alone remember the (maybe) long forgotten details of the biological process required to effectively design and utilize these tools. We certainly understand and are here to help!

Starting next week, we'll release the first post in our newest blog series - CRISPR 101 - a companion series to our popular Plasmids 101 articles. These posts are created to educate all levels of scientists and provide a resource for some of the basic principles driving CRISPRs and genome editing technology.

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Topics: Genome Engineering, Plasmids 101, CRISPR, CRISPR 101

Science as Art

Posted by Emma Markham on Mar 2, 2015 10:13:17 AM

Over the past decade there has been a shift in how science is perceived - where once science was only seen as a serious academic subject, it is now being appreciated on an aesthetic level as well. Beautiful microscope images which traditionally languished in the pages of thick textbooks are now liberated to adorn the walls of universities and the front pages of newspapers. Science is now being adapted to inspire to a new generation.

Daniel Stoupin is a PhD scientist and photographer based at the University of Queensland, whose outstanding marine photography has attracted much attention over the past few years, including an article in the BBC Wildlife Magazine. It is easy to see how these incredible images are attracting so much attention.

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Topics: Fun

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Posted by Kendall Morgan on Feb 24, 2015 2:50:00 PM

CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in mind. “We had not managed to create a system to allow us to reliably activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively activating them with the CRISPR system was a large one.  The question for her then was this: Can you engineer CRISPR-Cas9 activators that work well enough on any gene that they could be used by people with little bioengineering expertise?

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Topics: Plasmid Technology, CRISPR, pooled libraries

Management for Scientists: Delegating is Key

Posted by Joanne Kamens on Feb 20, 2015 3:30:00 PM

This is the fourth in a 5 part series on Management for scientists. Subscribe to the Addgene Career Advice Posts here.

Once you are responsible for managing others you will only be successful in your role if you become a master at delegating tasks and responsibilities. The manager is not expected to DO all the work she is expected to make sure the work gets done and done well. Involving your team effectively is now your new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage you don’t have time for that and you won’t be as productive. For example, the many resources avaliable on the Addgene website didn't come into being through the actions of a single person but hrough the coordinated efforts of many individuals.

Check out Joanne's Reddit AMA

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Topics: Career, Management for Scientists

CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

Posted by Guest Blogger on Feb 18, 2015 10:09:22 AM

The following post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9" from the Journal of Visualized Experiments (JOVE). The video publication by Stuart Orkin and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell lines. Below Bauer and Canver discuss the motivations behind this research.

 

Using CRISPR/Cas9 for Targeted Genomic Deletions

We were inspired to produce intrachromosomal deletions based on the experiments of Kim and colleagues using zinc finger nucleases to harness non-homologous end joining repair (NHEJ) [1]. Our initial work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised by the efficiency of the method. One observation was an inverse relationship between deletion size and frequency [3].

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Topics: Genome Engineering, Lab Tips, CRISPR, Protocols

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