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Melina Fan

Melina is the Director of Scientific Outreach and a co-founder of Addgene. She received her PhD from Harvard University studying diabetes and obesity. She loves that her job brings her in contact with scientists from around the world working on everything from cancer to biofuels.

Recent Posts

CRISPR 101: Validating Your Genome Edit

Posted by Melina Fan on Jul 30, 2015 10:30:00 AM

You’ve created your gRNA expression construct and used Cas9 to introduce it into your target cells. Hooray! You’re ready to begin reading out data, right? Almost. In this blog post we’ll explain how to verify that your cells were appropriately edited. We’ll also cover the basic techniques for detecting insertion, deletion, and mutation events.

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Topics: Genome Engineering, CRISPR, CRISPR 101

Plasmids 101: Multicistronic Vectors

Posted by Melina Fan on Sep 9, 2014 4:20:00 PM

Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple or bidirectional promoters, or the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA transcripts for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from the same mRNA. We've discussed promoters before so in this blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work, and how to get started with them.

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Topics: Plasmid Technology, Plasmids 101

Save Time and Money by Making Your Own Competent Cells

Posted by Melina Fan on Jan 28, 2014 10:10:58 AM

We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using a little-known reagent for streamlining the transformation step.

Read on to find protocols and tips that you can use in your own lab.

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Topics: Plasmid How To, Inside Addgene, Lab Tips

The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

Posted by Melina Fan on Jan 8, 2014 10:10:30 AM

Addgene was founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions. This has given us a unique window into technology trends in the life sciences.

In this post, we'll give you an inside look at the Top 10 plasmid technologies distributed through Addgene over our first 10 years.

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Topics: Plasmid Technology, Hot Plasmids, Scientific Sharing, Inside Addgene, Plasmid Kits

From our Table to Yours: An Inside Look at Lunch at Addgene

Posted by Melina Fan on Nov 27, 2013 9:54:00 AM

Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My co-founders actually were my family (Kenneth Fan is my brother and Benjie Chen is my husband), and we were joined by Judy Tsai, a friend from graduate school. The four of us would eat together every day, sharing ideas for the company, but also talking about sports, movies, and just about anything.

As our organization grew, we continued this tradition. We now have over 30 full-time employees, so we created one long lunch table and we eat together Harry Potter-style.The shared lunches create a culture of openness and trust that comes from caring about your colleagues on a personal level, and employees often cite lunch as one of their favorite parts about working at Addgene.

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Topics: Fun, Inside Addgene

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