Have you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed searching for research articles? Well then, my scientist friend, do I have a handy tool for you.
This post was contributed by guest blogger Joe Mellor, Founder and CEO of seqWell Inc.
Plasmids and PCR products are the bread and butter of molecular biology labs the world over. Scientists have traditionally used Sanger sequencing to validate these constructs, as the relatively low cost and quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology labs. Recent and rapid advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA, as well as the advent of extremely fast, cheap and accurate sequencing (“reading”) of DNA, have changed our collective thinking about the feasible size and scope of projects in many labs. However, the high costs of sample preparation for high-throughput next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes like plasmid validation.
At seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids from all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using this technique in our partnership with Addgene to sequence large numbers of plasmids.
We've been updating our plasmid validation processes to make it easier for you to find what you need in the repository, but we're also making it easier than ever to deposit with Addgene. Our plasmid deposit process can be broken down into three simple steps:
- Send Plasmid Information to Addgene
- Materials Transfer Agreement approval (MTA)
- Send Plasmids Addgene and Quality Control
Steps 2 and 3 are usually very easy - our tech transfer team will communicate with your university directly to make sure the MTA is taken care of and, once we have all of your plasmid data, we’ll send you prepaid shipping materials (i.e. a deposit kit) with instructions on how to send liquid DNA or bacterial streaks of your plasmid back to us. Our scientists will contact you if any issues arise during the QC process. As the depositing scientist, you will have the most involvement with step 1 and, while data entry can be a chore, we’ve made it easier than ever to send us your plasmid information with the Deposit Spreadsheet.
Topics: Inside Addgene
Last year was an exciting one for Addgene as we introduced our long-awaited viral service, but we haven’t forgotten about our plasmids! Now, we’re improving our quality control processes using next-generation sequencing (NGS) services provided by seqWell. This new QC process will bring you full sequence data for new plasmids entering the repository. Read on to learn more about how this process works and what you can expect to see on our plasmid pages.
Topics: Inside Addgene
Instead of spending time and money producing virus from select vectors in the repository yourself, you can now order ready-to-use virus directly from Addgene! As part of our new Viral Service, we’re distributing lentivirus (with many CRISPR tools included among the preps that are currently available) and adeno associated virus (AAV, primarily chemogenetics tools for now but with optogenetic tools coming soon). The viral preparations undergo rigorous quality control testing at Addgene meaning they come ready made to accelerate your research.