MXS Chaining

Posted by Leila Haery on Feb 7, 2017 10:30:00 AM

High-throughput cloning, in a nutshell, is the systematic combination of different genetic sequences into plasmid DNA. In high throughput cloning techniques, although the specific sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning procedure can be used to incorporate each element into the final construct. This strategy can be used to build vectors with diverse functions, and thus, is used in many biological fields. In synthetic biology for example, high-throughput cloning can be used to combine the functions of different genetic elements to generate non-natural tools such as novel biological circuits or sensors. Given the expanding palette of fluorescent proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure and protein localization, as well as cellular processes like gene expression and cell migration (Sladitschek and Neveu, 2015).

Read More >

Topics: Fluorescent Proteins, Plasmid Cloning

Michael Davidson and Roger Tsien Commemorative Travel Awards

Posted by Tyler Ford on Jan 5, 2017 10:55:23 AM

 

UPDATE (February 1, 2017): THE TRAVEL AWARD IS NOW CLOSED - WE WILL EMAIL ALL APPLICANTS SHORTLY TO INDICATE WE HAVE RECEIVED THEIR APPLICATIONS AND WILL NOTIFY AWARDEES IN EARLY MARCH 2017

To commemorate their innumerable contributions to the development and use of fluorescent protein tools and their dedication to scientific sharing, Addgene is opening applications for the Michael Davidson and Roger Tsien Commemorative Travel Awards. These $2,000 USD awards will be open to any masters students, PhD students, or postdocs traveling to an academic conference in 2017 who can demonstrate that fluorescent proteins have or will have an impact on their research.

Read More >

Topics: Fluorescent Proteins

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Posted by Guest Blogger on Sep 8, 2016 10:30:00 AM

This post was contributed by guest blogger, Luke Lavis, a Group Leader at the Janelia Research Campus, Howard Hughes Medical Institute.

Chemistry is Dead, Long Live Chemistry!

The discovery of green fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists were no longer beholden to chemists and (expensive) synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing fluorophore fusions on their own. Subsequent advances in fluorescent proteins have replicated many of the properties once exclusive to small-molecules: red-shifted spectra, ion sensitivity, photoactivation, etc. These impressive advances lead to an obvious question: In this age of GFP and its ilk, why should cell biologists talk to chemists?

Read More >

Topics: Imaging, Fluorescent Proteins

Editor's Choice, July 2016

Posted by Tyler Ford on Aug 5, 2016 11:00:00 AM

To better highlight the great content contributed by our bloggers each and every month, we've decided to start an "Editor's Choice" series. Each month, I'll summarize the most popular post of the month and point out one or more additional posts that deserve a peek in case you missed them.

Read More >

Topics: CRISPR, Fluorescent Proteins, Editor's Choice

Special Delivery: Fluorophore Targeting for FRET Studies

Posted by Guest Blogger on Jul 19, 2016 10:30:00 AM

This post was contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and Women’s Hospital.

Biochemists often struggle to understand how a protein of interest actually behaves. How large is it? What parts of it move when you feed it substrate or add an essential cofactor? How many binding partners does it have and how do they come off and on in a cellular environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical path to answers.

Read More >

Topics: Lab Tips, Fluorescent Proteins

Addgene blog logo

Subscribe to Our Blog