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If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an amplification protocol with your deposit. This is because repeated amplifications of pooled libraries can lead to issues such as recombination and loss of plasmid diversity, so ...
The triple T’s of biology are transformations, transfections, and transductions! In this blog we will discuss all things transductions. If you’ve ever wondered how they are different from or similar to the other Ts, we have your answers. If you know the basics but are here ...
What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 21 additions we've made to our viral vectors repository this year (and it's only March!)
Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through how we produce both our AAV preps and our lentiviral preps.
Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among the most important are the inverted terminal repeats (ITRs). ITRs are the ...
The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally, delivery is achieved through the use of ...
Calcium is arguably one of the most important metal ions in biology (sodium and potassium are up there too!). Calcium is fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural component of bones and teeth. It is an enzyme ...
