Plasmids 101: Aptamer Fluorophores

Posted by Eric J. Perkins on Apr 11, 2017 10:30:00 AM

What is an Aptamer?

Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, developed methods for selecting and amplifying RNA sequences that could bind very specifically to target molecules. Using a technique called systematic evolution of ligands by exponential enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity to a wide range of non-nucleotide targets. These RNA molecules, which could bind their targets with high specificity and affinity, were eventually called aptamers, from the Latin aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the next two decades, these nucleotide-based ligand binders would prove to be highly adaptable tools.

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Topics: Plasmids 101, Fluorescent Proteins

Plasmids 101: SunTag and Fluorescent Imaging

Posted by Mary Gearing on Mar 28, 2017 10:30:00 AM

Quick Announcement from the Plasmids 101 Team: In preparation for the release of Addgene's Fluorescent Protein eBook - our next couple of plasmids 101 posts will gain a healthy, fluorescent glow. Stay tuned for more fluorescence-based Plasmid 101 posts in the coming weeks!

In biology as in life, more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology, the Vale and Weissman labs took this biological lesson and created a system to amplify fluorescent signals. Named for the "stellar explosion SUperNova," SunTag can help you turn up the brightness in your fluorescent imaging experiments.

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Topics: Hot Plasmids, Plasmids 101, Fluorescent Proteins

Plasmids 101: Environmental Plasmids

Posted by Jessica Welch on Jan 31, 2017 10:30:00 AM

Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy in research, but where did these tools come from and why do they exist in nature? Read on to learn more about environmental plasmids, and how they’ve helped us develop molecular biology tools for the lab.

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Topics: Plasmids 101, Microbiology

Plasmids: Simplified

Posted by Tyler Ford on Jan 20, 2017 10:26:04 AM

We recently worked with YouTuber, Tom McFadden, to create a Plasmid Rap and introduce newcomers to the world of plasmids. As part of this process, we sent Tom a primer on plasmids and some of the ways that they can be used. We present this primer to you now with the hope that you can use it to introduce plasmids to any novice molecular biologist. You can find much more information about plasmids and their uses in our Plasmids 101 series. Happy reading!

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Topics: Plasmids 101

Plasmids 101: Gateway Cloning

Posted by Maria Soriano on Jan 12, 2017 10:30:00 AM

When facing a cloning project, scientists are no longer limited to traditional restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given set of resources, time, and experimental needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular as a rapid and highly efficient way to move DNA sequences into multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone a gene of interest into a variety of expression systems. Keep reading to learn more about the Gateway cloning method and its advantages.

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Topics: Plasmids 101, Plasmid Cloning

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